Description
Immortalized human umbilical vein endothelial cell line established (HUVEC) by electroporation of pIRES-hTERT-hygr.
Suggested Medium
MCDB131 medium supplemented with 10% fetal bovine serum, 0.03g/L endothelial cell growth supplement (ECGS), 5ug/mL heparin, and 10mM L-glutamine.
Quality Control
HUEhT-1 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
epithelial-like
Subculture
trypsinization with 0.025% trypsin-0.265mM EDTA
Shippment
HUEhT-1 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
HUEhT-1 Cell Line products are for research use only.