Description
HT-29 cell line was derived from colon. STR Profile is available.
Adherent Suspension
monolayer
Suggested Medium
RPMI1640 with L-glutamine (300mg/L), 25mM HEPES and 25mM NaHCO3, 90%; heat inactivated 10% FBS.
Quality Control
This cell line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins.
Organism
Human - female, 44 years old, Caucasian
Histopathology
adenocarcinoma
Subculture
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37C) until the cells detach (about 10min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Shippment
Fresh cells are shipped at Room Temperature
Warranty
All our cell products are for research use only. They are not authorized for human, in vitro diagnostic procedures or for therapeutic procedures.
