Description
HR5 cells were obtained after transfection of HeLa cells with plasmids coding for a reverse tetracycline-controlled transactivator encoding a nuclear localisation sequence (rt TA-nls) and transferring G418 resistance. This transactivator (a fusion between the Tet repressor and the activating domain of the herpes simplex virus VP16 transcription activation domain) can stimulate the expression of a reporter gene up to 10E5-fold. HR5 is the parent cell line for HR5-CL11.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
HR5 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Epithelial-like
Shippment
HR5 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
HR5 Cell Line is for research use only
