Description
The cell line HMT-3522 S2, also known as HMT-3522/gt1, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. A mutation at codon 179 of the p53 gene was detected during establishment of the parent line at passage 25?30. The subline S1 is a continuation of this line from passage 34 (ECACC catalogue no. 98102210) growing without collagen. Subline S2 has been isolated by culturing S1 cells from passage 118 in serum-free media without epidermal growth factor (EGF). A third subline, T4-2 (ECACC catalogue no. 98102212) was established from S2 at passage 238 and is the only tumorigenic line out of the 3 sublines. S2 and T4-2 grow on collagen-coated flasks. HMT-3522 S2 is near-diploid, but an evolution of karyotype has been observed by regular chromosomal analyses. The expression of cytokeratin 13, 14, 17 and 18 and vimentin has been reported. Overexpression of mRNAs for EGF-R, TGF-alpha, TGF-beta and c-erb-B2 compared to subline S1 has been shown. In addition, expression of p53 mRNA has been reported. The cells have been tested positive for EGF receptor but negative for estrogen receptor. Growth is inhibited by addition of EGF. The decreased growth factor requirements developed simultaneously with amplification and overexpression of c-myc protooncogene. Publications using this cell line must refer to the original paper (Briand et al., In Vitro Cell Dev Biol 1987; 23181). Any sublines created must include the name ?HMT-3522? in the designation, but a short name may be used in the same paper. Further references are available on request.
Adherent Suspension
Adherent
Suggested Medium
DMEM / Ham's F12 (11) + 2mM Glutamine + 250ng/ml insulin + 10µg / ml transferrin +10E-8M Sodium selenite + 10E-10M 17 beta-estradiol + 0.5µg/ml hydrocortisone + 5µg / ml ovine prolactin
Quality Control
Cell lines were tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Epithelial-like
Shippment
The cells are shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Our cell products are for research use only. They are not authorized for human, in vitro diagnostic procedures or for therapeutic procedures.
