Description
HMT-3522 S1, also known as HMT-3522/wt, is a subline that has been derived from HMT-3522. The parent line was established from a benign breast tumour of a 48 year old woman and has undergone spontaneous malignant transformation. It was grown on collagen in serum-free media without antibiotics from explantation onwards. A mutation at codon 179 of the p53 gene was detected during establishment of the parent line. HMT-3522 S1 is a continuation of this line from passage 34 growing without collagen. Subline S2 (ECACC catalogue no. 98102211) has been isolated by culturing S1 cells from passage 118 in serum-free media without epidermal growth factor (EGF). The third subline, T4-2 (ECACC catalogue no. 98102212) was established from S2 at passage 238 and is the only tumorigenic line in nude mice out of the 3 sublines. S2 and T4-2 grow on collagen-coated flasks. HMT-3522 S1 is near-diploid, but an evolution of karyotype has been observed by regular chromosomal analyses. The expression of cytokeratin 13, 14, 17 and 18 and vimentin has been reported. Expression of EGF-R, c-erb-B2, N-myc, c-ras-Ha1, c-ras-Ki2 and N-ras was shown to fluctuate slightly with passage number. The cells have tested negative for estrogen receptor, c-erbA1, c-erb-A2, int-2 or hst-1. During propagation growth factor requirements decreased simultaneously with amplification and overexpression of c-myc protooncogene. In a three-dimensional basement membrane culture assay the phenotypic characteristics of normal breast tissue in vivo are recapitulated. This cell line has remained non-tumorigenic for more than 400 passages. Publications using this cell line must refer to the original paper (Briand et al., In Vitro Cell Dev Biol 1987; 23181). Any sublines created must include the name HMT-3522 in the designation, but a short name may be used in the same paper.
Quality Control
Cell lines were tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
