Description
The HL-60 cell line was derived from peripheral blood leukocytes obtained by leukopheresis of a 36-year-old Caucasian female with acute promyelocytic leukemia. It was among the first long-term suspension cultures of human myeloid leukaemic cells to be established. Approximately 10% of HL-60 cells spontaneously differentiate and differentiation can be stimulated by polar planar compounds butyrate, hypoxanthine, phorbol myristic acid (PMA, TPA), dimethylsulfoxide (DMSO, 1% to 1.5%), actinomycin D, and retinoic acid.
Adherent Suspension
Suspension
Suggested Medium
RPMI 1640 + 2mM Glutamine + 10-20% Foetal Bovine Serum (FBS).
Quality Control
HL60 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
HL60 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
HL60 Cell Line is for research use only
