Description
Human Neural Stem Cells from Glow Biologics are differentiated from a human induced pluripotent stem cell line (hiPSC), which is generated using mRNA reprogramming technology from Human Dermal Fibroblasts (HDF). The monolayer HDF-hiPSC are efficiently converted to neural epithelium using HPSC Neural Induction Medium, a serum-free medium for rapid and efficient neural induction of human pluripotent stem cells (hPSCs). Synergistic inhibition of glycogen synthase kinase 3 (GSK3) and transforming growth factor b (TGF-b) differentiates HDF-hiPSCs to homogenous neural stem cells (NSC) in the presence of human leukemia inhibitory factor (LIF) within 7 days.The derived NSC are characterized by immunofluorescence with antibodies specific to nestin and SOX2. The cell population is highly pure: >90% of cells express Nestin and >80% of cells are SOX2 positive.
Suggested Medium
It is recommended to use Human Neural Stem Cells Medium for culturing HNSC in vitro.
Quality Control
HiPSC-derived Neural Stem Cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi.
Shippment
The frozen stem cells will be shipped using dry ice.
Storage
On receipt, immediately transfer the frozen cells to liquid nitrogen (-180 °C)
Warranty
For Research Use Only
