Description
The cell line HCT 116 is hemizygous for p300 and is expressed from a single allele. p300 knockout cells were obtained by targeting exon 2 of the EP300 gene using the targeting construct pIRESbeta-Geo-p300KO. Rescue clones were obtained by co-transfecting pcDEF Flag-p300 expression vector with pPGK-Puromycin construct, as described by Iyer et al. 2004.
Adherent Suspension
Adherent
Suggested Medium
McCoy's 5a medium modified, with 10% foetal bovine serum and 2mM L-Glutamine
Quality Control
HCT 116 p300 KO Rescue Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Polygonal epithelium
Shippment
HCT 116 p300 KO Rescue Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
HCT 116 p300 KO Rescue Cell Line is for research use only
