Description
The cell line HCT 116 is hemizygous for p300 and is expressed from a single allele. p300 knockout cells were obtained by targeting exon 2 of the EP300 gene using the targeting construct pIRESbeta-Geo-p300KO, as described by Iyer et al. 2004.
Adherent Suspension
Adherent
Suggested Medium
McCoy's 5a medium modified, with 10% foetal bovine serum and 2mM L-Glutamine.
Quality Control
HCT 116 p300 KO [D10] Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Polygonal epithelium
Shippment
HCT 116 p300 KO [D10] Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
HCT 116 p300 KO [D10] Cell Line is for research use only
