Description
HCT 116 BRCA2 -/- [42] human colorectal carcinoma cells were generated by homologous recombination to study the role of BRCA2 in DNA repair and to identify additional functions of the BRCA2 gene. These cells show phenotypes consistent with previous reports, including loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and elevated sensitivity to the DNA-damaging agents phleomycin and Parp1 inhibitors. Clone 42 was generated at the same time as clone 46 cited in Xu et al., 2014. J Pathol. 2014 Nov;234(3)386-97. The originating laboratory have advised they observed no difference in culture phenotype between clone 42 and clone 46.
Adherent Suspension
Adherent
Suggested Medium
McCoy's 5a medium modified, with 10% foetal bovine serum and 2mM L-Glutamine
Quality Control
HCT 116 BRCA2 -/- [42] Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Polygonal epithelium
Shippment
HCT 116 BRCA2 -/- [42] Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
HCT 116 BRCA2 -/- [42] Cell Line is for research use only
