Description
Established from a squamous cell carcinoma (SCC) of the floor of the mouth (20mm-40mm) of a 40 year-old female patient. STNMP stage III, well differentiated, node positive tumour. Mutant p53, codon 266 exon 8, G to T; wild type K-, N-, and Ha-ras. This cell line is highly responsive to TGFbeta. H376 cells in culture scatter, i.e. the cells redistribute on the substrate, in response to Hepatocyte Growth Factor. Non-tumourigenic in athymic nude mice by subcutaneous injection and on injection into the floor of the mouth (orthotopic). Haplotype information A*03,A*24; B*27,B*35; Cw*04,Cw*12
Adherent Suspension
Adherent
Suggested Medium
DMEMHAMS F12 (11) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Quality Control
H376 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
H376 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
H376 Cell Line is for research use only
