Description
Established from a squamous cell carcinoma (SCC) of the tongue (20mm) of a 74 year-old male patient. STNMP stage I, well differentiated, node negative tumour. Mutant p53, codon 110 exon 4, G to A; the previously reported mutant Ha-ras status of this cell line codon 13, G to A, codon 61, A-G is under investigation. This cell line is highly responsive to TGF beta and undergoes epithelial to mesenchymal transition. It expresses high levels of TGF beta1. Fahey et al (1996). Tumourigenic in athymic nude mice on subcutaneous injection and non-tumorigenic on orthotopic injection. Haplotype information A*02,A*31; B*40,B*44; Cw*03,Cw*05
Adherent Suspension
Adherent
Suggested Medium
DMEMHAMS F12 (11) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Quality Control
H357 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
H357 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
H357 Cell Line is for research use only
