Description
Established from a squamous cell carcinoma of the floor of the mouth of an 82 year-old male patient. STNMP stage II, moderately differentiated, node positive tumour. Mutant p53, codon 176 exon 5, G to T and 373 exon 11, A to G; wild type K-, N- and Ha-ras. Tumourigenic in athymic nude mice on subcutaneous injection; on injection into the floor of the mouth, cells spread to lymphatics and lymph nodes. By short tandem repeat-PCR analysis the Y chromosome could not be detected in this cell line when tested at Glow Biologics. It cell line is a known phenomenon that SSC cell lines can lose their Y chromosome.
Adherent Suspension
Adherent
Suggested Medium
DMEMHAMS F12 (11) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate
Quality Control
H314 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Polygonal & spindle
Shippment
H314 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
H314 Cell Line is for research use only
