General information
Synonyms: H22-H8D8
Species: Mouse
Tissue: liver
Disease: Hepatocellular carcinoma of the mouse
Gender: N/A
Doubling Time: N/A
Culture Medium: DMEM+10%FBS
Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Not specified
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.