Description
The breast epithelial cell line fR5 was established in 1982 by infecting suspensions of primary milk cultures with wild-type SV40. The cells do not express Muc-1, also known as HMFG-1 antigen, which cell line is a mucin-like component of human milk fat globule membranes. They are negative for keratins 4, 6, 7, 8, 10, 13 14, 16, 17, 18 and 19. Although fR5 is morphologically different from fR2, they have probably been derived from a common precursor cell since the karyotypes are similar. Karyotype analysis revealed hypotetraploidy and several rearrangements involving chromosome 1 and 11 which are frequently found in breast carcinomas and lines derived from metastatic pleural effusions. fR5 cells display anchorage independent growth in soft agar and are positive for SV40 T-antigen. Early passages were non-tumourigenic in nude mice.
Adherent Suspension
Adherent
Suggested Medium
RPMI 1640 + 2mM Glutamine + 10µg/ml insulin + 5µg/ml hydrocortisone + 10% FBS
Quality Control
fR5 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
fR5 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
fR5 Cell Line is for research use only
