Description
A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A. Cells will not divide at 39°C (non-permissive temperature) and will grow at 33°C (permissive temperature). Electron microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39°C for 16 hours. Thcell line is abnormal condensation does not occur at 33°C or in the FM3A cell line at either temperature.
Adherent Suspension
Suspension
Suggested Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
FM3Ats C1.T85 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
FM3Ats C1.T85 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
FM3Ats C1.T85 Cell Line is for research use only
