Description
FLYRD18 has been developed together with FLYA13 for packaging of retroviruses, it is especially useful for in vivo gene delivery. Both cell lines have been derived from HT1080. FLYRD18 has been transfected with Moloney murine leukaemia virus cores with envelope glycoproteins of cat endogenous virus RD114. This cell line cell line is able to produce human complement-resistant retroviral vectors at titres consistently higher than 1x10,000,000i.u./ml. Addition of 4µg/ml blasticidin S and 10µg/ml phleomycin into the media may enhance the packaging function. No replication competent virus has been detected in this cell line.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
FLYRD18 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Epithelial-like
Shippment
FLYRD18 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
FLYRD18 Cell Line is for research use only
