Description
The cell line F-36P has been established from a 65 year old male patient, who was diagnosed with refractory anaemia with excess blasts (RAEB), a subtype of myelodysplastic syndrome (MDS). This non-lymphoid leukemia line was obtained from the patient's pleural fluid at time of progressed to overt leukemia. F-36P is cytokine-dependent requiring granulocyte-macrophage colony-stimulating factor (GM-CSF) or Interleukin 3 (IL-3). Cells have been shown to synthesize haemoglobin when GM-CSF or IL-3 is substituted by erythropoietin (Epo). They have been reported to be positive for leukocyte common antigen (CD45) and some multilineage markers such as CD13, CD33 and CD34, but are negative for T- and B-cell antigens and mature myelomonocytic antigens. Due to their reaction with some monoclonal antibodies recognising erythroid and platelet glycoproteins it has been suggested that F-36P has a multilineage phenotype.
Adherent Suspension
Suspension
Suggested Medium
RPMI 1640 + 2mM Glutamine + 5ng/ml rh IL-3 + 5% Foetal Bovine Serum (FBS).
Quality Control
F-36P Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Lymphoblastoid
Shippment
F-36P Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
F-36P Cell Line is for research use only
