Description
Peripheral blood lymphocytes from a healthy male Tunisian, immortalised by EBV transformation. The cell line contains the following deletion, at the homozygous state, in the human immunoglobulin heavy locus (IGH on chromosome 14 at 14q32.33), of 6 IGHC genes on both chromosomes 14, deletion I (del G1-EP1-A1-GP-G2-G4). The Gm-Am genotype is Gm5*; - ; - A2m2/Gm5*; - ; - A2m2 (Gm b*; - ; - A2m2/Gm b*; - ; - A2m2). Gm allotypes are indicated in the order G3m; G1m; G2m. The absence of G1m and G2m allotypes due to the deletion of the IGHG1 and IGHG2 genes is indicated by (-). The EZZ deletion (deletion I), which encompasses 160 kb involves two highly homologous regions located 13 kb downstream of IGHG3 and 13 kb downstream of IGHG4.
Adherent Suspension
Suspension
Suggested Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
EZZ (TOU II-4) Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
EZZ (TOU II-4) Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
EZZ (TOU II-4) Cell Line is for research use only
