Description
The drug-resistant cell line EMT6/MTXR was derived from the mouse mammary tumour cell line EMT6/P by growth in increasing concentrations of methotrexate. EMT6/MTXR did not hyperexpress P-glycoprotein and did not show cross-resistance to other drugs of the multi-drug resistance (MDR) phenotype. It is recommended to culture the cells without drug on resuscitation until first passage or growth has been fully established.
Adherent Suspension
Adherent
Suggested Medium
MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1µg/ml methotrexate + 15% Foetal Bovine Serum (FBS). Resuscitate in drug-free medium; add drug once growth is fully established
Quality Control
EMT6/MTXR Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Epithelial-like
Shippment
EMT6/MTXR Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
EMT6/MTXR Cell Line is for research use only
