Description
The drug resistant cell line EMT6/AR10.0 was derived from the mouse mammary tumour cell line EMT6/P by continuous growth in increasing concentrations of doxorubicin. It was reported to show reduced intracellular drug accumulation and hyperexpression of P-glycoprotein. It is recommended to culture the cells without drug after resuscitation until the first passage.
Adherent Suspension
Adherent
Suggested Medium
MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA)+ 10.0µg/ml doxorubicin + 10% Foetal Bovine Serum (FBS).
Quality Control
EMT6/AR10.0 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Epithelial-like
Shippment
EMT6/AR10.0 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
EMT6/AR10.0 Cell Line is for research use only
