Description
E11 cell line is a clone of the cell line SSN-1 and is persistantly infected with a C-type retrovirus (SnRV). It is susceptible to piscine nodavirus strains belonging to different genotypes (SJNNV, RGNNV, TPNNV and BFNNV - striped jack, red spotted grouper, tiger puffer and barfin flounder nervous necosis viruses repectively). E11 is highly permissive to nodavirus infection and production. An advantage of E11, over the parental line SSN-1, is its steady, faster growth to confluency and longer stability in monolayer cultures for 2 weeks at 25°C or 4 weeks at 20°C and its reproducible, clear CPE characterised by cytoplasmic vacuole formation followed by intensive disintegration.
Adherent Suspension
Adherent
Suggested Medium
L15 + 5% Foetal Bovine Serum (FBS)+ 2mM Glutamine
Quality Control
E11 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
E11 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
E11 Cell Line is for research use only
