Description
The dysplastic oral keratinocyte cell line DOK was isolated from a piece of dorsal tongue from a 57 year old man. After a squamous-cell carcinoma was removed from the patient, who had been a heavy smoker, the remaining dysplasia was removed subsequently and used to initiate primary cultures leading to the establishment of DOK. The degree of dysplasia in the patient was described as mild to moderate. The cells show some stratification in confluent cultures and contain a keratin profile similar to the original dysplasia. Growth is stimulated by hydrocortisone and inhibited by cholera toxin. Expression of p53 is increased and a 12 bp in-frame deletion of the p53 gene (codon 188-191) could be identified. DOK cells are non-tumourigenic in athymic nude mice.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2mM Glutamine + 5µg/ml Hydrocortisone + 10% Foetal Bovine Serum (FBS).
Quality Control
DOK Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
DOK Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
DOK Cell Line is for research use only
