Description
DMS 153 was isolated from the liver of a 44 year-old male patient at autopsy who had been treated with cytoxan and methotrexate. The cells were identified as small cell carcinoma of the lung (SCLC) and secrete moderate quantities of calcitonin (CT) and calcitonin gene related peptide (CGRP). Production of adrenocorticotropin (ACTH), bombesin (BN), 17 beta-estradiol and oxytocin-neurophysin (OT-NP) has also been reported. The expression of cell surface antigens Leu-7, HLA-DR, My 23 (defined by AML-2-23 monoclonal antibody), the AML-1-99 defined antigen and CR3 receptor (defined by the OKM antibody) has been shown. DMS 153 cells produce receptors for BN and epidermal growth factor (EGF) and are tumourigenic in athymic, nude mice.
Adherent Suspension
Semi-adherent aggregates
Suggested Medium
Waymouth's MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).
Quality Control
DMS 153 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Tissue
Lung (liver metastasis)
Shippment
DMS 153 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
DMS 153 Cell Line is for research use only
