Description
This cell line is likely to be derived from olfactory dark (horizontal) basal cell. Keratin is expressed in this cell line.
Suggested Medium
MCDB153 with 0.1mM ethanolamine, 0.1mM phosphoethanolamine,10ng/ml EGF,5ng/ml bFGF, 5ug/ml insulin,10ug/ml bovine pituitary extract and 50ug/ml transfe
Quality Control
DBC1.2 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Tissue
olfactory epithelium
Morphology
epithelial-like
Subculture
0.1% (4,000-5,000 USP units/ml) crystalline trypsin in Ca, Mg-free PBS, and is stopped with 0.1% soybean trypsin inhibitor (Sigma type I-S).
Shippment
DBC1.2 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
DBC1.2 Cell Line products are for research use only.
