Description
The cell line COR-L23/R cell line is a multi-drug resistant (MDR) sub-line derived from the parent line COR-L23 after treatment with doxorubicin (adriamycin). The P-glycoprotein negative cell line is cross resistant to daunorubicin, vincristine and rhodamine 123. Drug resistance is caused by enhanced drug efflux that can be inhibited by addition of buthionine sulphoximide (BSO), an inhibitor of glutathione synthesis. COR-L23/R contain reduced levels of glutathione and glutathione-S-transferase activity compared to the parent line. MDR seems to be connected with overexpression of multi-drug resistance-associated protein (MRP) which is discussed as a drug transporter.
Adherent Suspension
Adherent
Suggested Medium
RPMI 1640 + 2mM Glutamine + 0.2µg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS). Start without drug. Add drug after first passage and then add at all subsequent passages.
Quality Control
COR-L23/R Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
COR-L23/R Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
COR-L23/R Cell Line is for research use only
