Description
Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific for non-structural protein NS3 (helicase domain). Thcell line cell line is antibody binds to a major conformational epitiope located at the carboxyl-terminal end of the NS3 region that is recognised by approximately ~90% from HCV-infected patients. CM3.B6 can be used in immunochemistry, HCV protein purification and HCV typing. CM3.B6 and B12.F8 were derived from the same patient as confirmed by STR profiling.
Adherent Suspension
Suspension
Suggested Medium
RPMI-1640 + 2mM L-Glutamine + 1% Non-Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS) + 20U/ml Interlukin-6 (IL-6).
Quality Control
CM3.B6 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Small rounded cells
Shippment
CM3.B6 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
CM3.B6 Cell Line is for research use only