Description
The fish line CHSE/F was derived from Common bluegill embryo. It is susceptible to various fish viruses including infectious hematopoietic necrosis virus; viral haemorrhagic septicaemia virus; spring viraemia of carp virus; and infectious pancreatic necrosis virus. Glow Biologics has been advised by the CCLV that this cell line is derived from Lepomis macrochirus not Oncorhynchus tshawytscha as originally reported. This has been confirmed by DNA sequencing at Glow Biologics. The CCLV have changed the cell line name to CHSE/F to indicate this. The line is used in viral diagnostics and gives better results than BF-2, a commonly used cell line of Lepomis macrochirus. The sequence of both lines (BF-2 and CHSE/F possess the same nucleotide exchange but are different in culture behaviour and in virus propagation.
Adherent Suspension
Adherent
Suggested Medium
MEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 120mg/l Sodium Pyruvate (NaPy) + 10% Foetal Bovine Serum (FBS).
Quality Control
CHSE/F (formerly known as CHSE-214) Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Fibroblast-like
Shippment
CHSE/F (formerly known as CHSE-214) Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
CHSE/F (formerly known as CHSE-214) Cell Line is for research use only
