Description
A subclone of the parental CHO line originated by Puck in 1957. The cells have an absolute requirement for L-proline. The cells have been adapted for growth in Sigma CHO Protein Free Medium (C5467). They have been cryopreserved in Sigma Cell Freezing Medium - Serum Free (C2639).
Adherent Suspension
Suspension
Suggested Medium
Sigma CHO-protein free medium + 4mM Glutamine. Cells should be frozen in either Sigma Cell Freezing Medium - Serum Free (C2639) with 10% DMSO or Sigma CHO Protein Free Medium (C5467) with 7.5%-10% DMSO
Quality Control
CHO (PROTEIN FREE) Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
CHO (PROTEIN FREE) Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
CHO (PROTEIN FREE) Cell Line is for research use only
