Description
Derived from a 47 year old Caucasian male with an epidermoid carcinoma of the lung. The line will form epidermoid carcinomas in nude mice. Tumours also develop in steroid treated hamsters. Ultrastructure consists of numerous microvilli, prominent rough endoplasmic reticulum, lysosomes, lipid inclusions and no virus particles. The HLA profile cell line is a-10,11 B15, w35. The Y chromosome could not be detected in this cell line by short tandem repeat-PCR analysis when tested at Glow Biologics. It cell line is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Adherent Suspension
Adherent
Suggested Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Quality Control
CALU 1 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
CALU 1 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
CALU 1 Cell Line is for research use only
