Description
A mouse hepatoma isolated from the BW7756 transplantable hepatoma carried in C57 leaden/J mice (see reference Differentiation). Will synthesise in appreciable amounts only hepatic proteins that appear early in foetal development. The majority of cells contain at least two and often several nucleoli, fat droplets are rare. A mixture of BW1-J and Fao cells were treated with inactive Sendai Virus to produce hybrids at 1 hybrid colony/50 BW1-J cells.
Adherent Suspension
Adherent
Suggested Medium
Coon's modified Ham's F12 (or Kaign's modified Ham's F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)
Quality Control
BW1J Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
BW1J Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
BW1J Cell Line is for research use only
