Description
The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia. The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at Glow Biologics.
Adherent Suspension
Suspension
Suggested Medium
RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Quality Control
BHL-89 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Tissue
Lymph node, auxillary
Morphology
Lymphoblastoid
Shippment
BHL-89 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
BHL-89 Cell Line is for research use only
