Description
The cell line BF-2 has been derived from a trypsinised suspension of pooled caudal portions of the trunk of 1 year old fingerlings of Bluegill fry (Lepomis macrochirus). Cells have been reported to be susceptible to a variety of fish viruses including lymphocystis virus, viral haemorrhagic septicaemia virus (VHSV), infectious pancreatic necrosis virus (IPNV) and tadpole edema virus (TEV) of frogs. The cell monolayer is confluent after 48-56 hours using a 14 split ratio. Catalogue no. at CCLV (depositor) is RIE 193.
Adherent Suspension
Adherent
Suggested Medium
MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA)+ 120 mg/l Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Quality Control
BF-2 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Fibroblast-like
Shippment
BF-2 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
BF-2 Cell Line is for research use only
