Description
The first human, trophoblastic endocrine cell type to be maintained in continuous culture. It was initiated from a malignant gestational choriocarcinoma of the foetal placenta. The cells secrete placental hormones including chorionic gonadotrophin (hCG), polypeptide hormones, lactogen, estrogenic and progestational steroids, estrone, estradiol, estriol and progesterone. A high rate of hCG Synthesis has been shown. Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. Due to the high glycolytic activity the cells have to be sub-cultured or supplied with fresh media every 24-48 hours to prevent glucose exhaustion. BeWo has the same DNA profile as JEG3; JEG3 was established by serial cloning of BeWo (see Pattillo & Gey 1968 PMID 4299001).
Adherent Suspension
Adherent
Suggested Medium
Ham's F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
BeWo Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
BeWo Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
BeWo Cell Line is for research use only
