Description
BEAS-2B cells were derived from normal bronchial epithelium obtained from autopsy of non-cancerous individuals. Cells were infected with a replication-defective SV40/adenovirus 12 hybrid and cloned. Squamous differentiation can be observed in response to serum. Thcell line is ability can be used for screening chemical and biological agents inducing or affecting differentiation and/or carcinogenesis. The cell line has been applied for studies of pneumococcal infection mechanisms. BEAS-2B was described to express keratins and SV40 T antigen. Subculturing the cells before confluency is necessary as confluent cultures rapidly undergo squamous terminal differentiation. This material is cited in a U.S and / or other Patent (U.S. Pat. 4,885,238) and may not be used to infringe the patent claims.
Adherent Suspension
Adherent
Suggested Medium
BEGM, also known as LHC-9 with modification (available from Clonetics Corporation, CC3171 (BEBM) plus additives CC4175 or BEGM Bullet Kit, CC3170); Note Additives supplied contain gentamycin which has been omitted for culture at ECACC; media is serum-free.
Quality Control
BEAS-2B Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Epithelial-like
Shippment
BEAS-2B Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
BEAS-2B Cell Line is for research use only
