Description
An 8-bromodeoxyuridine resistant mutant of mouse L cell fibroblasts. B82 lacks thymidine kinase and was isolated by virtue of resistance to 8-bromodeoxyuridine. Glow Biologics has two catalogue entries for the B82 cell line which differ in that they were deposited by different groups B82 and B82(Hamprecht). Several other cell lines in the Glow Biologics catalogue have been derived from B82(Hamprecht).
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 1 mM 8-bromodeoxyuridine
Quality Control
B82 (Hamprecht) Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Tissue
L cells fibroblasts
Shippment
B82 (Hamprecht) Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
B82 (Hamprecht) Cell Line is for research use only