Description
B12.F8 Cell Line, Human B-lymphocyte cell line, immortalized with Epstein-Barr Virus (EBV), derived from a patient with chronic Hepatitis C (HCV type 1). Cell line produces human monoclonal antibody specific to the native HCV nucleoprotein N3. Thcell line cell line is antibody binds to native HCV nucleoprotein with no observable reactivity for the NS3, NS4 and NS5 regions. Thcell line cell line is antibody identifies a major B cell epitope within the immunodominant nucleoprotein amino terminal subregion (aa. 34-45). B12.F8 can be used in immunochemistry, HCV protein purification and HCV typing. CM3.B6 and B12.F8 were derived from the same patient as confirmed by STR profiling.
Adherent Suspension
Suspension
Suggested Medium
RPMI-1640 + 2mM L-Glutamine + 1% Non-Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS) + 20U/ml Interleukin-6 (IL-6).
Quality Control
B12.F8 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Small rounded cells
Shippment
B12.F8 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
B12.F8 Cell Line is for research use only
