Description
The cell line b.End3 has been established from brain endothelial cells of SV129 mice. Immortalisation has been carried out by infection of primary cells with retrovirus coding for the Polyoma virus middle T-antigen. b.End3 cells are positive for endothelial specific proteins (PECAM-1, Endoglin, MECA-32, Flk-1) tested by FACS. Inflammatory cytokines induce the expression of proteins such as ICAM-1, VCAM-1 and E-selectin. Injection into mice causes development of haemangiomas.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2mM Glutamine + 5µM 2-Mercaptoethanol (2ME) + 1mM Sodium Pyruvate (NaP) + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Quality Control
b.End3 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Endothelial-like
Shippment
b.End3 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
b.End3 Cell Line is for research use only
