Description
This AKR-2B cell line has the capacity to be activated to produce murine leukaemia virus (MLV). Mice of the high leukaemic inbred strain AKR regularly contain high levels of MLV throughout most of their post-natal life. However, studies of the AKR mouse embryo indicated that they contain very little, or no, infectious virus. Cell lines were found to produce virus only after several weeks in culture. Virus-negative cell lines can be induced to initiate synthesis of MLV (Virology 1971). Multiple transforming growth factors (TGFs) have been isolated from the serum-free conditioned media and acid-ethanol cell extracts of non-transformed AKR-2B cells.
Adherent Suspension
Adherent
Suggested Medium
McCoy's 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
AKR-2B Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
AKR-2B Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
AKR-2B Cell Line is for research use only
