Description
The ABSa15 cell line is derived from the calcified branchial arches of a gilthead seabream and is capable of extracellular matrix mineralization. Gene expression analysis is suggestive of pre-chondrocyte lineage. Extracellular matrix mineralization occurs on supplementation with calcium chloride, ?-glycerophosphate and ascorbic acid. Mineral deposits can be detected as early as two weeks of treatment and increase for up to four weeks. ABSa15 is useful for investigating the mechanism of cell differentiation, extracellular matrix mineralization and also the screening of mineralogenic molecules.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Quality Control
ABSa15 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Tissue
Branchial arches (calcified cartilage)
Morphology
Epithelial-like (with a polygonal morphology at confluence)
Shippment
ABSa15 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
ABSa15 Cell Line is for research use only
