Description
Hybrid generated by fusion of bromodeoxyuridine-resistant mutant of rat glioma cell line C6 and the 6-thioguanine-resistant mouse L cell fibroblast line A9 using inactivated Sendai virus. Cells formed from such a fusion are multipolar and extend small processes.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 µM aminopterin and 16 µM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days
Quality Control
54SCC2 Cell Line was tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Tissue
Fibroblastic/glioma
Shippment
54SCC2 Cell Line will be shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
54SCC2 Cell Line is for research use only
