Description
Cells will differentiate into adipocytes once confluent which takes approximately 10 days. Once confluent, cells should be grown in DMEM and 10% FCS and 5 micrograms/ml insulin. Media changes should take place every 48 hours. We would like to manage customer expectations with regard to the potential of the current 3T3 cell line stocks to differentiate into adipocytes. If you intend to use the cells for adipocyte differentiation please note When cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g. 2 to 5 weeks, and the proportion of the population which differentiates can be limited. If you have previously used 3T3 cells from an alternative source we cannot guarantee the differentiation performance will be the same. We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.
Adherent Suspension
Adherent
Suggested Medium
DMEM + 10% Newborn Calf Serum (NCS) + 2mM Glutamine.
Quality Control
Cell lines were tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Morphology
Fibroblast-like
Shippment
The cells are shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Our cell products are for research use only. They are not authorized for human, in vitro diagnostic procedures or for therapeutic procedures.
