Description
Transformed with sheared human Ad5 DNA. Sensitive to human adenoviruses and adenovirus DNA. Can be used to isolate transformation defective host-range mutants of Ad5 and for titrating human adenoviruses. This is a hypotriploid human cell line. The modal chromosome number was 64, occurring in 30% of cells. The rate of cells with higher ploidies was 4.2%. The der (1)t(1;15) (q42;q13), der(19)t(3;19)(q12;q13),der(12)t(8;12) (q22;p13) and four other marker chromosomes were common to most cells. Five other markers occurred in some cells only. The marker der(1) and M8 (or Xq+) were often paired. There were four copies of N17 and N22. Noticeably in addition to three copies of X chromosomes, there were paired Xq+ and a single Xp+ in most cells. The Ad insert was shown to consist of a colinear segment from nucleotides 1 to 4344 integrated into chromosome 19 (19q13.2). Expression of an unusual cell surface receptor for vitronectin has been reported. This is composed of the integrin beta-1 subunit and the vitronectin receptor alpha-v subunit.
Adherent Suspension
Adherent
Suggested Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% FCS.
Quality Control
Cell lines were tested and found to be free of mycoplasma, bacterial, viruses, and other toxins. All cells were above 95% viability before freezing.
Shippment
The cells are shipped using dry ice.
Storage
Store the cell lines in liquid nitrogen vapor (less than -130°C)
Warranty
Our cell products are for research use only. They are not authorized for human, in vitro diagnostic procedures or for therapeutic procedures.
