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CRISPR Knockout Cell Line Service

Glow Biologics offers one-stop-solution custom knockout cell line services using multi host cell lines ranging from tumor cell line to immortalized cell lines. 100% quality guaranteed.

Contact Us

  • Tel: 1-917-242-6320
  • Fax: 1-917-242-6531
  • Email:
  • Address: 580 White Plains Rd, Tarrytown

Custom Gene Expression Cell Lines

Custom CRISPR Gene Knockout (KO) Cell Line Service

Glow Biologics has developed our unique CRISPR/Cas9 gene knockout platform to accurately knockout the designated genes. CRISPR allows for efficient and flexible gene knockout at competitive cost compared with traditional TALEN (transcription activator-like effector nuclease) and ZNF (zinc finger nuclease). Our scientists are experienced to provide single or double gene knockout using CRISPR/Cas9, from gRNA construction to transfection and single clone generation of a wide range of host cell lines, like HEK293T, HeLa, HCT116, HepG2, A549 and other difficult-to-transfect immortalized cell lines.

Service Content:





Gene Knock out


(Single or Double genes)

· gRNA sequence (3-5 groups)

· Single cell clone

· QC & project report

~3-4 months




Ø 100% knockout monoclonal clones: We guarantee to provide the 100% KO single cell clones to our customers.

Ø Professional technical support: Our scientists have rich CRISPR genome editing and cell culture experiences.

Ø One-stop service is provided from gRNAs construction, cell transfection, single clones selection to sequencing analysis, our customers only need to provide the target genes and host cell line information.

Ø High success rate and low off-target rate: 3-5 groups of gRNAs are applied for design and verification; We also provide off-target analysis and detection.

Ø Fast delivery & full transparency: the knockout cell lines are delivered within 3 months.

Service Process:




  Project Evaluation

Phase: gRNA Vector Construction

Phase : Cell Transfection and Monoclonal Clone Selection


· Cell line’s amenability

· Target gene analysis


· gRNA design
design 3 ~ 5 groups

· gRNA vector construction

· gRNA target activity detection

· Vector Transfection

· Single Cell Clone screening

· Amplification culture

· Sequencing Validation

· Positive clone cryopreserved



~2 weeks

~10-14 weeks



    gRNA sequence

· Single cell clone line

· Sequencing data

· Project report


Single Gene Knock Out Cell Line Generation Service Case:

Custom Human xx Knockout Cell Line in Swiss-3T3

Vector Map:





PCR Result:


KO Sequencing Result:


For quote, please fill in the online form and email to info@glowbiologics.com.

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